In vitro Regeneration of Arabidopsis thaliana from Cultured Zygotic Embryos and Analysis of Regenerants
Identifieur interne : 003989 ( Main/Exploration ); précédent : 003988; suivant : 003990In vitro Regeneration of Arabidopsis thaliana from Cultured Zygotic Embryos and Analysis of Regenerants
Auteurs : R. S. Sangwan [France] ; Y. Bourgeois [France] ; F. Dubois [France] ; B. S. Sangwan-Norreel [France]Source :
- Journal of Plant Physiology [ 0176-1617 ] ; 1992.
English descriptors
- KwdEn :
- 2,4-D, 2iP, Arabidopsis thaliana, BAP, BM, CIM, GM, IAA, K, MES, NAA, SIM, TIBA, ploidy level, regeneration, zygotic embryo culture.
Abstract
Summary: Zygotic embryos of Arabidopsis thaliana, ecotypes C24, Landsberg-erecta and Columbia, were cultured in vitro to investigate regeneration. A range of growth regulators, carbon sources and media were tested in conjunction with the initial exposure of cultures to light. Fertile plants were regenerated from embryo-derived calli with several auxin and cytokinin combinations from all ecotypes tested. Ecotypes differed in their regeneration proficiency. Efficient regeneration occurred when embryos were plated first on the callus induction medium (BM1) with high auxin and low cytokinin, and then transferred to shoot-inducing medium (BM3) with high cytokinin and low auxin. Regeneration frequency was more than 85 %, and 5 to 15 shoots per explant were obtained in ecotype C24. Phloridzin increased regeneration efficiency. Infrequent somatic embryos were observed with either 2,4-D (1 mg/L) alone or in combination with kinetin (0.5 mg/L), but the embryos did not develop into plantlets. Flow cytophotometry and chromosome counts of shoot tip cells suggested that 95 % of the regenerants were diploid, morphologically normal and fertile, as were their progeny. It is concluded that zygotic embryos may be a good source for both efficient regeneration and transformation.
Url:
DOI: 10.1016/S0176-1617(11)80794-7
Affiliations:
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A range of growth regulators, carbon sources and media were tested in conjunction with the initial exposure of cultures to light. Fertile plants were regenerated from embryo-derived calli with several auxin and cytokinin combinations from all ecotypes tested. Ecotypes differed in their regeneration proficiency. Efficient regeneration occurred when embryos were plated first on the callus induction medium (BM1) with high auxin and low cytokinin, and then transferred to shoot-inducing medium (BM3) with high cytokinin and low auxin. Regeneration frequency was more than 85 %, and 5 to 15 shoots per explant were obtained in ecotype C24. Phloridzin increased regeneration efficiency. Infrequent somatic embryos were observed with either 2,4-D (1 mg/L) alone or in combination with kinetin (0.5 mg/L), but the embryos did not develop into plantlets. Flow cytophotometry and chromosome counts of shoot tip cells suggested that 95 % of the regenerants were diploid, morphologically normal and fertile, as were their progeny. It is concluded that zygotic embryos may be a good source for both efficient regeneration and transformation.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Hauts-de-France</li><li>Picardie</li></region><settlement><li>Amiens Cédex</li></settlement></list><tree><country name="France"><region name="Hauts-de-France"><name sortKey="Sangwan, R S" sort="Sangwan, R S" uniqKey="Sangwan R" first="R. S." last="Sangwan">R. S. Sangwan</name></region><name sortKey="Bourgeois, Y" sort="Bourgeois, Y" uniqKey="Bourgeois Y" first="Y." last="Bourgeois">Y. Bourgeois</name><name sortKey="Dubois, F" sort="Dubois, F" uniqKey="Dubois F" first="F." last="Dubois">F. Dubois</name><name sortKey="Sangwan Norreel, B S" sort="Sangwan Norreel, B S" uniqKey="Sangwan Norreel B" first="B. 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